PITPNM1 anticorps (C-Term)

N° du produit ABIN374422

Cet anticorps Chèvre Polyclonal détecte spécifiquement PITPNM1 dans WB et IF. Il présente une réactivité envers Humain, Souris, Boeuf (Vache) et Chien.

Aperçu rapide pour PITPNM1 anticorps (C-Term) (ABIN374422)

Antigène: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Reactivité: Humain, Souris, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PITPNM1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-PITPNM1 anticorps

Épitope: C-Term

Séquence: C-KARSISLKLD SEE

Specificité: This antibody detects NIR2 at C-term and is expected to recognise both reported isoforms.

Réactivité croisée (Details): Species reactivity (expected):Human, Dog, Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C-Terminus of the protein sequence according to NP_004901.2, NP_001124320.1. Genename: PITPNM1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 8000. Western blot: Customer's experiments gave a band at approx 160 kDa in lysates of a MouseMacrophage cell line after 2 μg/mL antibody staining (calculated MW of 130 kDa accordingto NP_004901.1). In ICC the customer found staining of vesicular cytoplasm in the samecell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PITPNM1

Antigène: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Autre désignation: NIR2

Sujet: NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.Synonyms: DRES9, Membrane-associated phosphatidylinositol transfer protein 1, NIR2, PITPNM, PITPNM1

ID gène: 9600

NCBI Accession: NP_004901

UniProt: O00562